The specialized items of equipment shown below are housed within the Department of BioMolecular Sciences and are readily available for departmental graduate students and post-doctoral associates to utilize in the course of their research activities.
The Department of BioMolecular Sciences at the University of Mississippi represents one of the most well equipped, state-of-the-art biomolecular sciences departments in the USA. Although not exhaustive, below is a list of some of the technology we have at our disposal.
NMR Facilities (School of Pharmacy shared resources)
- 600 MHz NMR Spectrometer: Varian; 3mm inverse probe; variable temperature control (vtc) magic angle probe for the measurement of NMR data with micrograms of sample; water suppression and solid state NMR
- 500 MHz NMR spectrometer: Agilient DD2; “OneNMR” probe for both high- and low-bands; equipped with Agilent 7620-AS autosampler with 96 sample capacity. 500 MHz NMR spectrometer: Agilient DD2; “OneNMR” probe for both high- and low-bands; equipped with Agilent 7620-AS autosampler with 96 sample capacity
- 500 MHz NMR spectrometer: Bruker Avance III HD; 5mm Prodigy CryoProbe400 MHz NMR spectrometer: Varian Mercury Plus; 3 mm broadband gradient probe; 50 sample changer
- TWO – 400 MHz NMR spectrometer: Bruker Avance III HDTM; 5mm BBFO probe
- 400 MHz NMR spectrometer: Bruker Avance DRX; 3 mm dual probe; vtc.
- 400 MHz NMR spectrometer: Bruker Avance DRX; 3 mm inverse probe with gradient; vtc.
Molecular Modeling Facilities
- Mississippi Center for Supercomputer Research (MCSR) currently has 3 supercomputers: Sequoia, Catalpa and Maple.
- 1 – Microway Dual Intel Xeon WhisperStation 2.5 GHz 12-core workstation
- 1 – Microway WhisperStation 2.5 GHz Xeon 8-core workstations
- 3 – Microway WhisperStation Intel Xeon L5520 Nehalem 2.26 GHz 16-core workstations
- 3 – Microway WhisperStation Intel Xeon L5420 Harpertown 2.5 GHz 8-core workstations
- 3 – Linux workstations
- 14- Windows computers
- Molecular Modeling Software – Schrödinger, Gold, and MOE software suites
- Campus licenses for CambridgeSoft ChemBioOffice and Gaussian.
(2) – Nikon Ti2-E motorized:
(1) Waters Alliance HT LC/MS System consists of:Waters 2790 Separations Module, the Waters ZQ™ Mass Detector (Waters 996 Photodiode Array (PDA) Detector, and FractionLynx™ software.
Five (5) Waters Alliance HPLC Systems: (UV, Diode Array, Evaporative Light Scattering detectors).
Four (4) Waters DeltaPrep 4000 series HPLC Systems: Equipped with preparative capability –
from hundreds of milligrams to grams per run, a PrepLC Controller, solvent delivery unit, an integral injector panel,
a Rheodyne 7725I injector, and Waters 996 Photodiode Array or Waters 2487 Dual Wavelength Absorbance Detector.
High Resolution Mass Spectrometry
Thermo Orbitrap Fusion Tribrid LC/MS with multiple fragmentation modes
including collisional induced disassociationg (CID), higher-energy
collisional dissociation (HCD), and electron transfer disassociation (ETD)
Dionex Ultimate 3000 UHPLC
Integrated autosampler/fraction collector and UV detector
Rudolph Autopol IV, a six-wavelength polarimeter equipped with stainless steel jacketed cells, for measuring optical rotations. Volumes accommodated range from 1.0 mL to 0.05 mL.
Fourier Transform Infrared Spectrophotometer
Bruker Vector33 FTIR, with NIR and MID FTIR capabilities, equipped with a fiber optics accessory for remote sensing, and an HYPERION IRScope with ATR capabilities for solid-phase analysis of chemically modified resins.
Hewlett-Packard 8453 Diode-Array Biochemical Analysis Spectrophotometer equipped with 7-cell thermostatted multicell transport accessory and dedicated water circulator. Biochemical Analysis software suite allows for kinetic analysis and real-time monitoring.
Perkin-Elmer CHN/SO Series II Analyzer
Elemenatal analyzer equipped with a 60 sample carosel for performing high-throughput elemenatal analyses.
COMPex Pro 102 KrF
Excimer laser with a maximum output of 400 mJ/pulse at 248 nm
In Vitro Core
TopCount NXTTM Microplate Scintillation and Luminescence Counter
The In Vitro Core’s TopCount NXTTM analyzes six at a time, and counts samples in 96- and 384-well microplates, as well as microcentrifuge tubes. The external plate stacker allows multiple assay plates for a high unattended counting capacity. The system automatically controls the counting chamber temperature to ensure a stable and reproducible counting environment. The TopCount NXTTM can be used for Cell proliferation, Receptor binding assay, Solid Phase RIA/LIA, Reporter gene assays, 51Cr release assays, HPLC fraction analysis and Scintillation Proximity Assays (SPA).
The FilterMate Harvester is configured for harvesting UniFilter microplates with built-in GF/B® or GF/C® filters for analysis on the TopCount Microplate Scintillation and Luminescence Counters.
The Tri-Carb 2900TR is a bench top liquid scintillation analyzer used to measuring small amounts of beta, gamma and alpha radioactivity. The Tri-Carb features QuantaSmart instrument software.
The epMotion machine is an automated pipetting system. It can dispense any quantity from 1-1000 μL in anywhere from 1-12 channels.
This machine is a fast-reading detector HTS reader. It includes two detectors which allows for simultaneous wavelength reading and reading below emission. It has stackers as well as internal temperature control for samples being loaded. It also has alpha screen capabilities and uses software to show plate readouts.
Thermo Scientific REVCO RB150 Incubator
This incubator is designed to retain CO2 levels internally for samples as well as keep them at temperature. Systems are designed to also “trap” external water. It offers thermal protection and insulation as well as spacious room for samples.
HERMLE LabNet Z323K Centrifuge
This high performance centrifuge has high speed rotors capable of producing up to 26,810 x g. Higher speed and g-force allow this centrifuge to be used in a biological lab setting. Parameters are set using easy turn knobs while their values are shown on large digital displays. Speed can be set and displayed in rpm or rcf. It has a radius-correction feature, and braking intensity can be set to ten different levels. There is also a refrigeration system internally, so heating or cooling can take place inside the centrifuge.
Olis DSM 20 CD for measurements of circular dichorism (CD).