3-Day Course Outline
Day 1: Botanical Authentication
- Taxonomy & Identification
- Plant taxonomy
- Botanical nomenclature
- Plant morphology
- Plant anatomy
- Herbarium/Voucher specimens
- Species identification
- Adulteration
- Adulterants and substitutes
- Authentication of Botanicals
- Organoleptic
- Macroscopy
- Microscopy
- GC-MS (technique and applications for botanicals/dietary supplements)
- HPTLC Technique and Lab Session – Overview of technology and entire process
- Preparation of samples and developing solvent (mobile phase)
- Plate marking
- Spotting a plate
- Placing plate in development chamber
- Conditioning development chamber
- Development of plate
- Visualization and interpretation
- Calculations of RF values
- Plate derivitization and detection
Day 2: Sample Preparation and Analysis
- HPTLC Vs. HPLC
- Comparison
- Advantages and disadvantages
- Sample preparation
- Types of samples
- Sample handling
- Sample preparation techniques
- Modern separation techniques (HPLC, UHPLC)
- Theory
- Comparison
- Practical applications
- Detection techniques (UV-VIS, ELSD, MS)
- Comparison
- Key applications for natural products
- Columns/Column chemistry
- Column dimensions
- Particle/Pore size
- Stationary phases
- Types of analysis
- Qualitative (Fingerprinting)
- Quantitative (Marker compounds)
- Method transfer
- Overview
- HPLC Lab Session
Day 3: Data Processing
- Data processing
- Retention time
- Peak shape
- Resolution
- Spectral data
- Peak homogeneity
- Mass analysis window
- Automated Custom Calculations
- Milligram per capsule/tablet (For Liquids, mg/ml)
- Weight per serving
- Weight per daily dose
- Acceptance Limits
- Within specification
- UV Spectral Matching (Libraries)
- Overview
- Theory
- Benefits
- Practical applications
- Mass Detection
- Overview
- Benefits
- Practical applications