Natural Products Training Laboratory

The University of Mississippi

3 Day Advanced Course

This course will provide attendees with advanced training in analytical techniques. This will include a practical training and a critical understanding of the laboratory-based techniques they will apply during their research projects.

The Complete HPLC/UHPLC & LC-MS or GC-MS or HPTLC course is for those who have basic knowledge of liquid or gas chromatography and would like to greatly enhance it, covers all the practical theory that need to know about liquid/gas chromatography and its related techniques including LC-MS or GC-MS and HPTLC.  Sample preparation, mobile phase optimization including gradient elution, calibration standards, method validation, data analysis for quantitation and qualitative analysis are discussed. It also looks at how to develop appropriate application specific HPTLC, GC-MS & LC-MS methods, the set-up and optimization of instrumentation.  Finally, the steps required to maintain and troubleshoot the system, problem chromatography and mass spectra are covered.


Learning Outcomes

Upon successful completion of the 3-day advanced course, the attendees will gain advanced understanding in the following areas depending on the following selected analytical technique:

  • LC
  • GC/Microscopy
  • HPTLC/Microscopy


These techniques will provide:

  1. Strategic planning of analytical techniques to apply to different types of samples including selection of the most appropriate technique/instrumentation for the analysis.
  2. Design an analytical work-flow to acquire data and process data from the chosen instruments.
  3. Knowledge of the importance of good chromatography
  4. How to select/optimize an appropriate mobile phase and develop gradient or isocratic elution methods.
  5. Selection of columns/TLC plates (stationary phase and dimensions)
  6. Operation and selection of appropriate detection methods
  7. Principles and components of the mass spectrometer
  8. Ample preparation, calibration standards and sample properties
  9. Logical steps of instrument troubleshooting

You will have the option to bring your own pre-approved sample* or have one furnished for you.

*Samples must be approved via email in advance of the course and must consist of at least 3g of dried material