Microscopy and Imaging Center

University of Mississippi


The SEM facility in the University of Mississippi’s School of Pharmacy has been supporting the UM community in various research and educational programs. The data obtained from the SEM facility has supported a large number of research publications, doctoral theses, grant applications as well as conference presentations listed below:

Publications utilizing data from JSM-7200FLV FE-SEM

I. Research publications

  1. Butreddy, A., Sarabu, S., Bandari, S., Dumpa, N., Zhang, F., Repka, M. A., & Repka, M. A. (2020). Polymer-Assisted Aripiprazole-Adipic Acid Cocrystals Produced by Hot Melt Extrusion Techniques. Crystal Growth and Design. doi:10.1021/acs.cgd.0c00020
  2. Butreddy, A., Sarabu, S., Dumpa, N., Bandari, S., & Repka, M. A. (2020). Extended release pellets prepared by hot melt extrusion technique for abuse deterrent potential: Category-1 in-vitro evaluation. International Journal of Pharmaceutics, 587, 119624. doi:https://doi.org/10.1016/j.ijpharm.2020.119624
  3. Chatterjee, R., Sajjadi, B., Chen, W.-Y., Mattern, D. L., Hammer, N., Raman, V., & Dorris, A. (2020a). Effect of Pyrolysis Temperature on PhysicoChemical Properties and Acoustic-Based Amination of Biochar for Efficient CO2 Adsorption. Frontiers in Energy Research, 8(85). doi:10.3389/fenrg.2020.00085
  4. Chatterjee, R., Sajjadi, B., Chen, W.-Y., Mattern, D. L., Hammer, N., Raman, V., & Dorris, A. (2020b). Impact of Biomass Sources on Acoustic-Based Chemical Functionalization of Biochars for Improved CO2 Adsorption. Energy & Fuels, 34(7), 8608-8627. doi:10.1021/acs.energyfuels.0c01054
  5. Parveen, A., Adams, J. S., Raman, V., Budel, J. M., Zhao, J., Babu, G. N. M., . . . Khan, I. A. (2020). Comparative Morpho-Anatomical and HPTLC Profiling of Tinospora Species and Dietary Supplements. Planta Med, 86(7), 470-481. doi:10.1055/a-1120-3711
  6. Sajjadi, B., Shrestha, R. M., Chen, W.-Y., Mattern, D. L., Hammer, N., Raman, V., & Dorris, A. (2020). Double-layer magnetized/functionalized biochar composite: Role of microporous structure for heavy metal removals. Journal of Water Process Engineering, 101677. doi:https://doi.org/10.1016/j.jwpe.2020.101677
  7. Wang, H., Dumpa, N., Bandari, S., Durig, T., & Repka, M. A. (2020). Fabrication of Taste-Masked Donut-Shaped Tablets Via Fused Filament Fabrication 3D Printing Paired with Hot-Melt Extrusion Techniques. AAPS PharmSciTech, 21(7), 243. doi:10.1208/s12249-020-01783-0
  8. Zhang, P., Shadambikar, G., Almutairi, M., Bandari, S., & Repka, M. A. (2020). Approaches for developing acyclovir gastro-retentive formulations using hot melt extrusion technology. Journal of Drug Delivery Science and Technology, 60, 102002. doi:https://doi.org/10.1016/j.jddst.2020.102002
II. Conference presentations/abstracts
  1. Srinivasan P, M. Almutari, N.R. Dumpa, S. Bandari, F. Zhang, M.A. Repka. Impact of polymeric carriers on the processability of theophylline nicotinamide pharmaceutical co-crystals using hot melt extrusion technology. AAPS 2019 PHARMSCI 360. San Antonio, Texas. Nov. 3-6.
  2. Bandari S, S. Sarabu, N.R. Dumpa, S. Ajjarapu, R. Kallakunta, S.N. Murthy, M.A. Repka. Novel application of hot melt extrusion paired fused deposition modeling 3D printing techniques in fabrication of ketoprofen films for topical delivery. AAPS 2019 PHARMSCI 360. San Antonio, Texas. Nov. 3-6.
  3. Dumpa N.R., N. Komanduri, S. Bandari, M.A. Repka. Sustained release floating tablets prepared by Fusion Deposition modeling (FDM) 3D printing. AAPS 2019 PHARMSCI 360. San Antonio, Texas. Nov. 3-6.
III. Thesis/Dissertation

  1. Chatterjee, R. (2020). Incorporation of Acoustic and Chemical Modifications to Biochar for Pollution Abatement. D. Thesis. University of Mississippi. https://egrove.olemiss.edu/etd/1844
  2. Mclain, K. (2020). Crabs from the Cane River Formation of Northern Louisiana: A study of Neozanthopsis americana and associated fauna. Honors College Senior Thesis. University of Mississippi. https://egrove.olemiss.edu/hon_thesis/1457

Publications utilizing data from JSM-5600 Scanning Electron Microscope

I. Research publications
  1. Adelli GR, Balguri SP, Bhagav P, Raman V, Majumdar S. Diclofenac sodium ion exchange resin complex loaded melt cast films for sustained release ocular delivery. Drug Delivery. 2017;24(1):370-9.
  2. Adenekan K, Hutton-Prager B. Sticky hydrophobic behavior of cellulose substrates impregnated with alkyl ketene dimer (AKD) via sub- and supercritical carbon dioxide. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2019;560:154-63.
  3. Almutairy BK, Alshetaili AS, Ashour EA, Patil H, Tiwari RV, Alshehri SM, et al. Development of a floating drug delivery system with superior buoyancy in gastric fluid using hot-melt extrusion coupled with pressurized CO₂. Pharmazie. 2016;71(3):128-33.
  4. Alshehri SM, Park J-B, Alsulays BB, Tiwari RV, Almutairy B, Alshetaili AS, et al. Mefenamic acid taste-masked oral disintegrating tablets with enhanced solubility via molecular interaction produced by hot melt extrusion technology. Journal of Drug Delivery Science and Technology. 2015;27:18-27.
  5. Alshehri SM, Tiwari RV, Alsulays BB, Ashour EA, Alshetaili AS, Almutairy B, et al. Investigation of the combined effect of MgO and PEG on the release profile of mefenamic acid prepared via hot-melt extrusion techniques. Pharmaceutical development and technology. 2017;22(6):740-53.
  6. Alshetaili AS, Almutairy BK, Tiwari RV, Morott JT, Alshehri SM, Feng X, et al. Preparation and evaluation of hot-melt extruded patient-centric ketoprofen mini-tablets. Current Drug Delivery. 2016;13(5):730-41.
  7. Alsulays BB, Kulkarni V, Alshehri SM, Almutairy BK, Ashour EA, Morott JT, et al. Preparation and evaluation of enteric coated tablets of hot-melt extruded lansoprazole. Drug Dev Ind Pharm. 2017;43(5):789-96.
  8. Alsulays BB, Park J-B, Alshehri SM, Morott JT, Alshahrani SM, Tiwari RV, et al. Influence of molecular weight of carriers and processing parameters on the extrudability, drug release, and stability of fenofibrate formulations processed by hot-melt extrusion. Journal of Drug Delivery Science and Technology. 2015;29:189-98.
  9. Angamuthu M, Nanjappa SH, Raman V, Jo S, Cegu P, Narasimha Murthy S. Controlled-release injectable containing Terbinafine/PLGA microspheres for Onychomycosis Treatment. Journal of Pharmaceutical Sciences. 2014;103(4):1178-83.
  10. Ashour EA, Majumdar S, Alsheteli A, Alshehri S, Alsulays B, Feng X, et al. Hot melt extrusion as an approach to improve solubility, permeability and oral absorption of a psychoactive natural product, piperine. Journal of Pharmacy and Pharmacology. 2016;68(8):989-98.
  11. Battu SK, Repka MA, Maddineni S, Chittiboyina AG, Avery MA, Majumdar S. Physicochemical Characterization of Berberine Chloride: A Perspective in the Development of a Solution Dosage Form for Oral Delivery. AAPS PharmSciTech. 2010;11(3):1466-75.
  12. Bhagurkar AM, Darji M, Lakhani P, Thipsay P, Bandari S, Repka MA. Effects of formulation composition on the characteristics of mucoadhesive films prepared by hot-melt extrusion technology. Journal of Pharmacy and Pharmacology. 2019;71(3):293-305.
  13. Bookwala M, Thipsay P, Ross S, Zhang F, Bandari S, Repka MA. Preparation of a crystalline salt of indomethacin and tromethamine by hot melt extrusion technology. European Journal of Pharmaceutics and Biopharmaceutics. 2018;131:109-19.
  14. Budel JM, Raman V, Monteiro LM, Almeida VP, Bobek VB, Heiden G, et al. Foliar anatomy and microscopy of six Brazilian species of Baccharis (Asteraceae). Microscopy Research and Technique. 2018;81(8):832-42.
  15. Chatterjee R, Sajjadi B, Chen W-Y, Mattern DL, Egiebor NO, Hammer N, et al. Low Frequency Ultrasound Enhanced Dual Amination of Biochar: A Nitrogen-Enriched Sorbent for CO2 Capture. Energy Fuels. 2019;33(3):2366-80.
  16. Chatterjee R, Sajjadi B, Mattern DL, Chen W-Y, Zubatiuk T, Leszczynska D, et al. Ultrasound cavitation intensified amine functionalization: A feasible strategy for enhancing CO2 capture capacity of biochar. Fuel. 2018;225:287-98.
  17. Dayan FE, Owens DK, Watson SB, Asolkar RN, Boddy LG. Sarmentine, a natural herbicide from piper species with multiple herbicide mechanisms of action. Frontiers in Plant Science. 2015;6(Journal Article):222.
  18. Dumpa NR, Sarabu S, Bandari S, Zhang F, Repka MA. Chronotherapeutic Drug Delivery of Ketoprofen and Ibuprofen for Improved Treatment of Early Morning Stiffness in Arthritis Using Hot-Melt Extrusion Technology. AAPS PharmSciTech. 2018;19(6):2700-9.
  19. Joshi VC, Khan IA, Sharaf MHM. Use of scanning electron microscopy in the authentication of botanicals. Pharmacopeial Forum. 2008;34(4):1075-8.
  20. Joshi VC, Srinivas PV, Khan IA. Rapid and easy identification of Illicium verum hook. f. and its adulterant Illicium anisatum Linn, by fluorescent microscopy and gas chromatography. J AOAC Int. 2005;88(3):703-6.
  21. Lalge R, Thipsay P, Shankar VK, Maurya A, Pimparade M, Bandari S, et al. Preparation and evaluation of cefuroxime axetil gastro-retentive floating drug delivery system via hot melt extrusion technology. International Journal of Pharmaceutics. 2019;566:520-31.
  22. Lu J, Cuellar K, Hammer NI, Jo S, Gryczke A, Kolter K, et al. Solid-state characterization of Felodipine-Soluplus amorphous solid dispersions. Drug Dev Ind Pharm. 2016;42(3):485-96.
  23. Manda P, Angamuthu M, Hiremath SR, Raman V, Murthy SN. Iontophoretic Drug Delivery for the Treatment of Scars. Journal of Pharmaceutical Sciences. 2014;103(6):1638-42.
  24. Maurya A, Nanjappa SH, Honnavar S, Salwa M, Murthy SN. Rapidly Dissolving Microneedle Patches for Transdermal Iron Replenishment Therapy. Journal of Pharmaceutical Sciences. 2018;107(6):1642-7.
  25. Maurya A, Rangappa S, Bae J, Dhawan T, Ajjarapu SS, Murthy SN. Evaluation of soluble fentanyl microneedles for loco-regional anti-nociceptive activity. International Journal of Pharmaceutics. 2019;564:485-91.
  26. McFall H, Sarabu S, Shankar V, Bandari S, Murthy SN, Kolter K, et al. Formulation of aripiprazole-loaded pH-modulated solid dispersions via hot-melt extrusion technology: In vitro and in vivo studies. International Journal of Pharmaceutics. 2019;554:302-11.
  27. Mididoddi PK, Repka MA. Characterization of hot-melt extruded drug delivery systems for onychomycosis. European Journal of Pharmaceutics and Biopharmaceutics. 2007;66(1):95-105.
  28. Modepalli N, Shivakumar HN, McCrudden MTC, Donnelly RF, Banga A, Murthy SN. Transdermal Delivery of Iron Using Soluble Microneedles: Dermal Kinetics and Safety. Journal of Pharmaceutical Sciences. 2016;105(3):1196-200.
  29. Mohammed NN, Majumdar S, Singh A, Deng W, Murthy NS, Pinto E, et al. Klucel™ EF and ELF polymers for immediate-release oral dosage forms prepared by melt extrusion technology. AAPS PharmSciTech. 2012;13(4):1158-69.
  30. Mohammed NN, Pandey P, Khan NS, Elokely KM, Liu H, Doerksen RJ, et al. Clotrimazole-cyclodextrin based approach for the management and treatment of Candidiasis – A formulation and chemistry-based evaluation. Pharmaceutical Development and Technology. 2016;21(5):619-29.
  31. Palem CR, Dudhipala NR, Battu SK, Repka MA, Rao Yamsani M. Development, optimization and in vivo characterization of domperidone-controlled release hot-melt-extruded films for buccal delivery. Drug Dev Ind Pharm. 2016;42(3):473-84.
  32. Pimparade MB, Vo A, Maurya AS, Bae J, Morott JT, Feng X, et al. Development and evaluation of an oral fast disintegrating anti-allergic film using hot-melt extrusion technology. European Journal of Pharmaceutics and Biopharmaceutics. 2017;119(Journal Article):81-90.
  33. Raman V, Budel JM, Zhao J, Bae J-Y, Avula B, Osman AG, et al. Microscopic characterization and HPTLC of the leaves, stems and roots of Fadogia agrestis – an African folk medicinal plant. Revista Brasileira de Farmacognosia. 2018;28(6):631-9.
  34. Raman V, Galal AM, Avula B, Sagi S, Smillie TJ, Khan IA. Application of anatomy and HPTLC in characterizing species of Dioscorea (Dioscoreaceae). Journal of Natural Medicines. 2014;68(4):686-98.
  35. Raman V, Galal AM, Khan IA. An Investigation of the Vegetative Anatomy of Piper sarmentosum, and a Comparison with the Anatomy of Piper betle (Piperaceae). American Journal of Plant Sciences. 2012;3(8):1135-44.
  36. Raman V, Raman V, Avula B, Avula B, Galal AM, Galal AM, et al. Microscopic and UPLC–UV–MS analyses of authentic and commercial yohimbe (Pausinystalia johimbe) bark samples. Journal of Natural Medicines. 2013;67(1):42-50.
  37. Raman V, Sagi S, Galal AM, Avula B, Viljoen A, Khan IA. Adulteration in commercial buchu dietary supplements: Analyses of commercial and authentic buchu samples and comparative studies of Agathosma betulina and Agathosma crenulata by microscopy and HPTLC. South African Journal of Botany. 2015;100:122-31.
  38. Raman V, Tabanca N, Demirci B, Khan IA. Studies on the floral anatomy and scent chemistry of titan arum (Amorphophallus titanum, araceae). Turkish Journal of Botany. 2017;41(1):63-74.
  39. Rutter T, Hutton-Prager B. Investigation of hydrophobic coatings on cellulose-fiber substrates with in-situ polymerization of silane/siloxane mixtures. International Journal of Adhesion and Adhesives. 2018;86:13-21.
  40. Vo AQ, Feng X, Morott JT, Pimparade MB, Tiwari RV, Zhang F, et al. A novel floating controlled release drug delivery system prepared by hot-melt extrusion. European Journal of Pharmaceutics and Biopharmaceutics. 2016;98(Journal Article):108-21.
  41. Vo AQ, Feng X, Pimparade M, Ye X, Kim DW, Martin ST, et al. Dual-mechanism gastroretentive drug delivery system loaded with an amorphous solid dispersion prepared by hot-melt extrusion. European Journal of Pharmaceutical Sciences. 2017;102(Journal Article):71-84.
  42. Ye X, Ye X, Patil H, Patil H, Feng X, Feng X, et al. Conjugation of Hot-Melt Extrusion with High-Pressure Homogenization: a Novel Method of Continuously Preparing Nanocrystal Solid Dispersions. AAPS PharmSciTech. 2016;17(1):78-88.
  43. Zhang J, Feng X, Patil H, Tiwari RV, Repka MA. Coupling 3D printing with hot-melt extrusion to produce controlled-release tablets. International Journal of Pharmaceutics. 2017;519(1-2):186-97.
    II. Conference presentations/abstracts
    1. Raman V, Budel JM, Lata H, Chandra S, Khan IA, ElSohly MA. Identification of Cannabis sativa using morpho-anatomical and microscopic characteristics. XIIth Brazilian Symposium of Pharmacognosy and XVIIth Latin American Symposium on Pharmacobotany. 2019.
    2. Parveen A, Budel JM, Raman V, Zhao J, Ganesh Babu NM, Ali Z, Khan IA. Comparative Morpho-anatomy and Qualitative Analysis of Tinospora 19th Annual International Conference on the Science of Botanicals. 2019.
    3. Raman V, Budel JM, Zhao J, Bae J, Avula B, Osman AG, Ali Z, Khan IA. Characterization of Fadogia agrestis leaf, stem and root by microscopy and HPTLC. 18th Annual International Conference on the Science of Botanical. 2018.
    4. Raman V, Tabanca N, Demirci B, Khan IA, 2016. Contributions to Morpho-Anatomy and Odor-Chemistry of ‘Carrion Flower’ (Amorphophallus titanum). 16th Annual International Conference on the Science of Botanicals & 5th Interim American Society of Pharmacognosy. 2016.
    5. Raman V, Bussmann RW, Khan IA, 2016. Identification of the True Bay Leaf and its Substitutes. 16th Annual International Conference on the Science of Botanicals & 5th Interim American Society of Pharmacognosy. 2016.
    6. Raman V, Khan IA. Calcium oxalate crystals in Dioscorea (Dioscoreaceae). American Society of Pharmacognosy Annual Meeting & 14th Annual Oxford International Conference on the Science of Botanicals. 2014.
    7. Raman V, Sagi S, Galal AM, Avula B, Khan IA. Comparative morpho-anatomy and HPTLC studies of leaves of Agathosma betulina and Agathosma crenulata. American Society of Pharmacognosy 2014 Annual Meeting & 14th Annual Oxford International Conference on the Science of Botanicals. 2014.
    8. Raman V, Avula B, Galal A, Wang YH, Khan IA. Identification of Yohimbe Bark (Pausinystalia johimbe) using Microscopy and UPLC-MS Techniques. 11th Annual Oxford International Conference on the Science of Botanicals. 2012.
    III. Ph.D. theses
    1. Adenekan K. Hydrophobization of Cellulose-Based Fibers for Packaging Applications with Alkyl Ketene Dimers (AKD) and Food-Grade Waxes via Supercritical Impregnation with Carbon Dioxide–Experimental and Thermodynamic Modeling Approaches. 2019.
    2. Almotairy A. Characterization of Drug-Loaded Milled Extrudate Particles Produced by Hot Melt Extrusion Technology for Dry Powder Inhalers. 2019.
    3. Almutairi M. Feasibility of AquasolveTM HPMC-AS Lg via Hot-melt Extrusion: Effect of Pressurized CO2 on Physico-mechanical Properties. 2018.
    4. Almutairy BK. Development and Characterization of Sustained-Release Formulations via Novel Hot Melt Extrusion Techniques. 2019.
    5. Alshehri SM. Versatility of hot-melt extrusion for dosage form design. 2015.
    6. Alshetaili AS. Solubility enhancement, mechanical properties and taste masking of poorly water soluble compounds by optimizing hot melt extrusion processing. 2015.
    7. Alsulays B. Novel applications and properties of hot melt extrudates.
    8. Ashour EA. Influence of novel techniques on solubility, mechanical properties and permeability via hot melt extrusion technology. 2015.
    9. Battu SK. Pre-formation and formulation Considerations for Transmucosal and Oral Delivery of Compounds of Natural Origin Produced Utilizing Hot-Melt Extrusion Technology.
    10. Bhagurkar AM. Novel Applications of Hot-Melt Extrusion Technology. 2018.
    11. Bookwala M. Crystalline Salt Formation of an Acidic Drug Indomethacin Using Hot Melt Extrusion Technology. 2018.
    12. Feng XIN. Evaluation and enhancement of physical stability of amorphous solid dispersions. 2016.
    13. Khan MM. Thermal Barrier Coatings on Paper Substrates.
    14. Lalge RM. Preparation and Evaluation of Cefuroxime Axetil Gastro-Retentive Floating Drug Delivery System for Improved Delivery via Hot-Melt Extrusion Technology. 2018.
    15. Lu J. Solubility Enhancement and Precipitation Inhibition of Poorly Water Soluble Compounds Utilizing Hot Melt Extrusion Technology. 2014.
    16. Maurya A. Effect of Solvent Interaction & Soluble Microneedles on Skin Permeability to Drug Molecules. 2019.
    17. McFall H. Formulation of aripiprazole-loaded pH-modulated solid dispersions via hot-melt extrusion technology.
    18. McFall H. Preparation and Evaluation of Aripiprazole-Loaded pH-Modulated Solid Dispersion via Hot-Melt Extrusion Technology. 2016.
    19. Mididoddi PK. Hot-melt Extruded Drug Delivery Systems for Onychomycosis and Other Fungal Infections. 2006.
    20. Modepalli N. Novel Delivery Systems For Iron Replenishment. 2015.
    21. Mohammed NN. Pharmaceutical formulation development utilizing hot melt extrusion and other techniques for development of immediate and controlled release dosage forms. 2012.
    22. Pimparade MB. Patient Friendly Formulations Utilizing Hot Melt Extrusion Technology. 2017.
    23. Pradhan AS. A Study on the Taste Masking Ability of Kollicoat® MAE 100-55 on Caffeine Citrate via Hot Melt Extrusion Technology.
    24. Shadambikar G. Preparation And Evaluation Of Valacyclovir Hydrochloride Ocular Inserts By Hot Melt Extrusion Technique. 2018.
    25. Shukla A. Mefenamic Acid–HPMC AS HG Amorphous Solid Dispersions: Dissolution Enhancement Using Hot Melt Extrusion Technology. 2019.
    26. Singh A. Design and development of drug delivery systems for immediate and sustained release utilizing hot melt extrusion. 2012.
    27. Srinivasan P. Investigation of Theophylline Nicotinamide Pharmaceutical Co-Crystals Utilizing Hot Melt Extrusion. 2019.
    28. Vo AQ. Approaches to Narrow Absorption Window Drugs Using Hot Melt Extrusion Technology.
    29. Ye X. Novel Application of Twin Screw Extruder on Production of Diverse Formulations. 2018.

    Helpful resources and external links

    Schedule a Service
    JEOL-JSM 7200F details
    SEM A to Z by JEOL (pdf download)
    SEM resources (JEOL)
    Oxford Instruments EDS
    Deben STEM detector
    Centaurus CL & BSE Detector
    Denton Vacuum Desk V sputter coater
    National Science Foundation
    UM Wins NSF Award for Electron Microscope
    University of Mississippi